摘要
体外实验发现,苄丙咯(bepridil.Bep)能显著抑制提纯的鼠脑钙调蛋白依赖性蛋白激酶(CaM—PKⅠ)的活力,其半抑制浓度约为0.3mmol·L^(-1),而尼莫地平(nimodinine,Nim)和尼卡地平(nicardipine;Nic)对该酶活力均无明显影响。实验结果表明,Bep对CaM—PKⅡ的抑制是通过擂抗CaM而实现的。
The effects of bepridil, ni-modipine and nicardipine on the activity of CaM - dependent protein kinase Ⅱ from mouse cerebrum were studied in vitro. Tt was found that bepridil significantly inhibitied the activity of CaM - PK Ⅱ with an IC50 value of 0. 3 mmol · L-1. but nimodipine and nicardipine did not basi-
cally influence the activity of the enzyme. The experimental results showed that the inhibitory effect of bepridil on the activity of CaM - PK Ⅱ was an antagonistic action of bepridil to CaM.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1992年第5期380-383,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助课题
关键词
苄丙咯
钙调蛋白
蛋白激酶
bepridil: nimodipine
nicardipine
CaM -dependent
protein ki-naseⅡ
