摘要
利用Heparin-Sepharose CL-6B(H-S-CL-6B)亲和层析法分离提纯了牛奶中的脂蛋白脂酶(LPL),并研究了茶叶多糖(TP)对该酶活力的影响。实验结果表明,1毫升反应体系中,160μg的TP能使LPL活力提高7%,并且能降低该酶对抑制剂如NaCl的敏感性,与肝素有相似的作用。
A method for the seperation and purification of the lipoprotein lipase (LPL) from bovine milk by affinity chromatography with Heparin-Sepharose CL-6B and studies on the effects of tea polysaccharide. (TP) on the enzyme activity were described. The experiment results showed that 160 μg/ml of TP could both increase the activity of LPL by 7% and reduce the sensitivity of LPL on inhibitor such as NaCl.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1992年第5期287-289,共3页
Journal of China Pharmaceutical University
关键词
脂蛋白脂酶
绿茶
茶叶
多糖
Tea leaves
Polysaccharide
Lipoprotein lipase
