摘要
本实验采用大鼠异位心脏移植模型,观察到:术前多次输注供体大鼠脾细胞可显著延长移植心存活时间,其作用呈供体抗原特异性,作用机理与受体大鼠T亚群改变及血清免疫抑制因子的产生有关。结果提示术前单用供体抗原难以达到移植心免疫耐受。
Inbred Wistar and SD rats were used as donor and recipient, respectively. Abdominal hetorotopic heart transplantation was adopted to study the effect of pretransplant transfusion of donor spleen cells(TDSC) on the prolongation of allograft survival time and its possible mechanism. The results showed: the mean survival time (MST)in the control group was 11.2 days, while those of the 1st(1 dose), 2nd (2 doses) 3rd (3 doses) TDSC group were 15.7, 20.5 and 38.4 days, respectively (p>0.05, p<0.01, p<0.01, respectively vs. control group); pretransplant transfusion of rat spleen cells other than Wistar twice did not prolong the survival time(MST=11.0). In the 1st TDSC group, CD4/CD8 ratio decreased slightly, the percentage of serum inhibiting MLR was 24.9%(p>0.05); but in the 2nd and 3rd TDSC groups, both of the CD4/CD8 ratio decreased (p<0.05 and p<0.01)and the percentage of serum inhibiting MLR increased (p<0.05 and p<0.01)significantly, furthermore, normal recipient spleen cells which absorbed the serum of rat of the 2nd TDSC group also presented significant suppression on MLR (p<0.05).The results confirmed that multiple TDSC can significantly prolong survival time of the allograft, which may be related to decreased CD4/CD8ratio and the serum inhibiting MLR mainly caused by donor antigen specific antibody to self-lympho-cytes. The results suggest that TDSC alone can not induce donor specific transplantation tolerance, but only hypoimmuuologic response to prolong survival time of the allograft.
出处
《中国循环杂志》
CSCD
1992年第5期445-448,共4页
Chinese Circulation Journal
关键词
心脏移植
免疫耐受
供体抗原
Heart transplantation
Specific tranplantation tolerance
Donor antigen
