摘要
目的 :探讨门静脉内注射供体脾细胞诱导移植肾的免疫耐受情况。方法 :实验组 Wistar大鼠在肾移植同时将经过预处理的供体 SD大鼠脾细胞注入门静脉 ,对照组则注入生理盐水 ,然后用环孢素 A治疗 1周 ,并以大鼠平均存活时间为标准比较两组结果。结果 :对照组平均存活( 1 0 .5± 2 .1 ) d,实验组为 ( 72 .2± 32 .0 ) d( P <0 .0 1 )。实验组在肾移植 60 d后 ,再移植 SD大鼠和Lewis大鼠的皮肤 ,发现 SD大鼠的皮肤不被排异 ,Lewis大鼠的皮肤出现排异。结论 :门静脉内注射供体脾细胞可诱导肾移植免疫耐受 ,并且这种耐受具有特异性。
Purpose:To study on the tolerance of renal allograft.Methods:We built the animal model on the specific tolerance of renal allorgaft.The wistar rats were divided into two groups.While the kidney transplantation was performed the donor rats SD spleen cells which were pretreated were injected into portal vein of the recipient rats wistar in experimental group, 0.9 % normal solution was injected into portal vein in control group.Results:The median survival peroid of the rats was ( 10.5 ± 2.1 ) days in control group,( 72.2 ± 32.0 ) days in experimental group (P< 0.01 ).60 days after the transplantation,the rats in the experimental group were transplanted with the skin from the SD rats or Lewis rats.Two weeks later,the SD rat′s skin were survived,the Lewis rat′s skin were dead.Conclusions:It was believed that the intraportal injection of donor spleen cells could induce recipients specific tolerance to renal allograft.
出处
《临床泌尿外科杂志》
2000年第3期122-124,共3页
Journal of Clinical Urology
关键词
肾移植
免疫耐受
动物实验
供体
脾细胞
Kidney transplantation Immune tolerance Animals,laboratory
