摘要
目的 研究过氯酸铵 (AP)对肺组织的损害作用 ,探讨AP有无致肺纤维化作用。方法 Wistar大鼠经气管内注入AP染毒一定时间后 ,取大鼠肺泡灌洗液 (BALF)和肺组织 ,测定细胞总数、肿瘤坏死因子 α(TNF α)、丙二醛 (MDA)、羟脯氨酸 (HYP)和胶原纤维等指标。结果 AP进入肺组织后能引起急性肺损伤、肺炎症反应 ;染毒后不同时间AP各组BALF中TNF α值均显著高于阴性对照组 (P <0 0 5 )。AP对MDA、HYP和胶原蛋白合成有一定影响 ;没有明显的肺纤维化病理变化。结论 AP对肺组织有急性损伤作用。
Objective To study the injury effect of ammonium perchlorate (AP) to lung and to explore whether AP can cause pulmonary fibrosis. Methods\ To detect the levels of cell counts, TNF α,MDA , HYP and the synthesis of collagen in BALF or rat lung after a certain time when rats were injected AP by intratracheal instillation. Results\ AP could bring about acute lung damage and inflammatory reaction. The levels of TNF α of different groups in different time were obviously higher than the normal control group( P <0 05). AP could affect the levels of MDA , HYP and the synthesis of collagen. But it had no obviously pathological change of pulmonary fibrosis. Conclusion\ There were acute injury effect about AP to lung, but this experiment could not make sure whether AP could cause pulmonary fibrosis.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第2期208-210,共3页
Journal of Hygiene Research
关键词
过氯酸铵肺纤维化
肿瘤坏死因子-A
羟脯氨酸
ammonium perchlorate, pulmonary fibrosis, tumor necrosis factor-α, hydroxyproline
