摘要
以反应温度、反应时间、酶的用量、底物浓度、pH值等为主要因素研究解脂假丝酵母脂肪酶水解乳脂肪的工艺条件,并利用响应面分析法(RSA)对水解条件进行了优化。得出最佳水解条件为底物浓度50.7%,pH7.5,加酶量317.3U/g,温度40℃。
The hydrolysis of milk fat catalyzed by lipase from Candida lipalytica was investigated. The temperature, the reaction time, the quantity of lipase, the concentration of milk fat and pH were taken as main factors. Response surface analysis was applied to determine the optimal conditions of hydrolysis.It was shown that the optimal conditions are the concentration of milk fat 50.7%, pH7.5, the quantity of lipase 317.3U/g,the temperature 40℃,the hydrolysis ratio of milk fat was 55.89% when lipase catalyzed milk fat hydrolysis was carried on 12 hours.
