摘要
壳聚糖是自然界中最丰富的多糖之一.壳聚糖酶能够降解壳聚糖生成壳寡糖,壳寡糖具有多种生物学特性,用途广泛.壳聚糖酶主要分布在微生物和一些植物中,理化性质较稳定.根据其底物特异性和断裂键的专一性可分为 、 、 三类.根据已测序壳聚糖酶的氨基酸序列相似性,壳聚糖酶分别属于糖苷水解酶46号、75号和80号.目前,只有2种壳聚糖酶的3级结构被测定.
Chitosan is one of the most abundant polysaccharides in nature. It consists of β-1, 4-linked D-glucosamine (GlcN) and N-acetyl-β-Dglucosamine (GlcNAc) residues. Chitosanases catalyze the hydrolytic breaking of its glucosidic bond and produce chitosan, which are widely applied for various purposes in medicine, agriculture, food and industry. Chitosanases found in microorganisms and some plants have stable physical and chemical properties. They are classified into three subclasses based on the substrate specificity and the cleavage positions of the partially acetylated chitosan. According to the amino acid alignment, most sequenced chitosanases belong to three-glycoside hydrolase families designated as number 46, 75 and 80. The three dimensional structures have been determined only for the chitosanases from Streptomycse sp. N174 and Bacillus circulans MH-K1.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2004年第2期229-236,共8页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省海洋局基金资助项目.
