3Polge C, Smith A U, Parkes A S. Revival of spermatozoa after vitri- fication and dehydration at low temperature [ J]. Nature, 1949, 164:666.
4Asahina E, Takahashi T. Cryopreservation of sea urchin embryos and sperm [ J ]. Development, Growth and Differentiation, 1979, 21:423-430.
5Adams S L, Hessian P A, Mladenov P V. Cryopreservation of sea urchin( Evechinus chloroticus)sperm [ J]. Reproductive Aquacul- ture, 2004,25:287-299.
6Anchordoguy T J,Crogwe J H,Griffin F J,et al. Cryopreservation of sperm from the marine shrimp Sicyonia ingentis [ J ]. Cryobiolo- gy,1988,25 (3) :238-243.
7Liliana S F, Carmen G P C, Jilla J, et al. Cryopreservation of sperm of red abalone ( Haliotis rufescens ) [ J ]. Journal of Shellfish Research, 2005,24 ( 2 ) : 415 -420.
8Suzuki T, Boediono A, Takagi M, et al. Fertilization and develop- ment of frozen-thawed germinal vesicle bovine oocytes by a one- step dilution method in vitro [ J ]. Crytobiology, 1996,33 : 515 - 524.
9Beattie G M, Crowe J H, Lopez A D, et al. Trehalose: a cryopro- tectant that enhances recovery and preserves function of human pancreatic islets after long- term storage [ J ]. Diabetes, 1997,46 (3) :519-523.
10Eroglu A, Russo M J, Bieganski R, et al. lntracellular trehalose improves the survival of cryoprescrved mammalian cells[ J]. Nature Biotechnology ,2000,18 : 163 - 167.