摘要
从粉正蚓成体中提取总RNA,以寡核苷酸Oligo(dT)20为引物合成cDNA第一条链;用RT PCR的方法扩增蚯蚓纤溶酶的cDNA,将其克隆到pUCm T载体上进行DNA序列测定.结果表明,所克隆的蚯蚓纤溶酶cDNA长度为747bp,其中编码区段为738bp,共编码246个氨基酸.将该序列与GenBank中其它的4种蚯蚓纤溶酶序列(LumbricusrubellusEFE III 1、Lumbricusrubelluslumbrokinase 3(1)precursor、Lumbricusbimastuslumbroki nase、Lumbricusrubelluslumbrokinase 1T4precursor)相比,同源性分别为99%,97%,89%,88%;与同源性最高的序列相比,本序列有7个碱基、3个氨基酸与该序列不同.
Earthworm fribrinolytic enzyme (EFE) cDNA was amplified by RT-PCR from adult worm of Lumbricus rubellus, and then cloned into pUCm-T vector. Sequence analysis revealed that the cDNA was 747 base pair, and that the encoding fragment was 738 base pair which encoded a protein of 246 amino acid residues. Comparison with four other EFE cDNA sequences (Lumbricus rubellus EFE-III-1、Lumbricus rubellus lumbrokinase-3(1) precursor、Lumbricus bimastus lumbrokinase、Lumbricus rubellus lumbrokinase-1T4 precursor) registered in GeneBank shows 99%,97%,89%,88% homology in DNA sequence respectively. There are seven base substitutions between the reported sequence and the Lumbricus rubellus EFE-III-1, those of which subsequently leads to change of three amino acids.
出处
《武汉大学学报（理学版）》
CAS
CSCD
北大核心
2004年第2期211-215,共5页
Journal of Wuhan University:Natural Science Edition
