摘要
收集蓝氏贾第鞭毛虫患者的新鲜粪便标本,用蔗糖密度梯度离心法纯化包囊。以包囊悬液感染哺乳期的长爪沙鼠,7d 后,在无菌条件下从受染乳鼠小肠上段获取本虫滋养体,用改良 TYI-S-33培养基,于37℃培养。培养后 d4虫体即在管壁形成密集的细胞单层,同日开始传代。将培养物置肉汤及血琼脂平板培养,未见细菌及霉菌污染,为纯培养。虫体的对数生长期为48～72h。在去除培基中的维生素一吐温80混合液成分后,虫体仍可生长繁殖。
Cyst suspension(6000 cyst/ml)of Giardia lamblia obtained and purified from a 62—years—old farmerliving in a rural area of Sichuan province was used to infect the suckling gerbils Meriones unguiculatus.Onday 7 after oral inoculation,trophozoites isolated aseptically from the upper intestinal tract of the infectedanimals were inoculated into modified TYI—S—33 medium and cultivated at 37℃.On day 4 of the culti-vation,the organisms became adapted to the in vitro cultivation,so that they formed a cellular monolayer onthe inner surface of the culture tube.No bacterial or fungal growth was detected by plating aliquots of theculture onto blood agar plates,or inoculating them into beef broth for cultivation at 37℃ for 4 days.Theperiod of logarithmic growth was from 48 to 72hr.After having become adapted to the in vitro environ-ment,the organisms could also grow and reproduce in the absence of vitamin—Tween 80 mixtures whichwere used at the start of the culture.So far,this axenic culture has been maintained for 6 months and 50subcultures have been made.
出处
《中国寄生虫病防治杂志》
CSCD
1992年第1期42-44,共3页
Chinese Journal of Parasitic Disease Control
