摘要
从两例人肺腺癌组织中分离出淋巴细胞。在体外重组白细胞介素-2条件下培养,在一段时间内出现持续增殖。病例A经45天培养淋巴细胞数扩增约267倍。病例B经32天培养淋巴细胞数扩增约87倍。肿瘤浸润性淋巴细胞在体外白细胞介素-2条件下培养,8～10天细胞毒活性明显提高,到15天达最高峰,维持约20天时间。
The potential utility of tumor infiltrating lymphocytes(TIL) in clinical trials has been suggested by murine exporiments showing these cells to be 50～100 times more powerful than LAK cells in the treatments of advanced metastic disease. A method for the expansion of human TIL from adenocarcinoma is described in this report, tumors were digested enzymatically to yield single ceil suspensions which were cultured in RPMI 1640 medium with 10% human AB serum and 400U/ml recombinant interleukin-2. TIL were expanded from 87 folds to 267 folds over a cultured period from 32～45 days. Furthermore TIL showed high cytotoxic activity against K_(562), an NK-sensitive myeloid leukemia cell line and AGZY_(83a), an NK-resistent human adenocarcinoma cell line.
