摘要
本实验用产生OH·体系(Fe^(2+)/抗坏血酸)直接损伤大鼠心肌线粒体,观察丹参素的保护作用。实验结果表明丹参素对线粒体ADP/O,RCR(respiratory control rate,呼吸控制率)、细胞色素氧化酶活性有保护作用,提示丹参素为良好的OH·清除剂。
The role of DS-182 in protection of cellular damages induced by oxygen free radicals was studied in rat cardiac mitochondria. The changes of cardiac mitochondrial enzyme (cytochrome oxidase) and P/O, RCR were used as indices of mitohondrial injury. The experimental animals were divided into three groups: control group, injury group (Fe^(2+)+ascorbic acid), and protection group (DS-182+Fe^(2+)+ascobic acid). Results obtained showed that incubation of mitochondrial suspension with ascorbic acid and Fe^(2+) resulted in decrease of activity of cytochrome oxidase, P/O (with α-ketoglutarate andsuccinate as substrate respectively) and RCR, which indicated that the structure and function of mitochondria were damaged. Danshensu (DS-182) can inhibit the decrease of cytochrome oxidase activity, P/O and RCR. Thus, DS-182 with acts as an effective scavenger for oxygen free radicals prevented the mitochondrial injury.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1992年第2期122-124,共3页
Chinese Journal of Pathophysiology
关键词
线粒体
心脏
丹参素
氧自由基
Mitochondria, heart
Danshensu (DS-182)
Oxygen free radicals
