摘要
改进病毒纯化方法,获得了较高纯度、较强侵染活性的提纯病毒制品。提纯病毒的产量为0.7—1.2mg/100g病叶,病毒粒体大小为720—750×14nm,SDS-聚丙烯酰胺凝胶电泳测得的外壳蛋白分子量为36000道尔顿,用提纯病毒免疫家兔得到较高特异性的抗血清,微量沉淀反应的效价为1/2048,间接ELISA法测出的感病叶汁液的最高稀释倍数为3200—6400倍。
Purified virus preparation of Maize Dwarf Mosaic Virus (MDMV) withhigh purity and infectivity was obtained by an improved procedure of puri-fication. The yield of purified virus was 0.7--1.2mg/100g infected tissue. Thevirus preparation had a typical ultraviolet absorption spectra of a filamentousvirus containing about 6% nucleic acid, A280/A260 was 0.63--0.80, Amax/Amin was 1.15. The particle size of purified virus was 720--750×14nm and themolecular weight of its coat protein was 36kd by SDS-PAGE. Antiserum witha titre of 1/2048 in micropreciptin test was specific to MDMV. Virus in in-fected tissue at the dilution of 3200--6400 folds could be detected with indi-rect ELISA.
出处
《中国病毒学》
CSCD
1992年第1期93-98,共6页
Virologica Sinica
