摘要
本文报告了采用聚合酶链反应(即PCR技术)检测人宫颈癌中人乳头瘤病毒16型(HPV16)的E_6/E_7相关序列。在正链引物(SP)和反链引物(ASP)的诱导下,以二步温控法聚合酶链反应对34例人宫颈癌组织DNA进行检测,发现宫颈癌中HPV16E_6/E_7相关序列存在的阳性率达67.6％(23/34)。本研究结果提示:HPV16可能在宫颈癌的发生发展过程中起重要作用,采用PCR技术选择性地检测HPV16的转化基因(E_6、E_7)对深入探讨宫颈癌发生的机理、早期发现宫颈癌患者等有重要意义。
Detection of the sequence related to E_6/E_7 of human papillomavirus type16 in Chinese cervical cancers using polymerase chain reaction (PCR) has beenreported in this paper. We have used the two-step protocol PCR to detectDNA sequence related to HPV16 E_6/E_7 in 34 cervical cancer tissues, by primingwith SP and ASP, the positive percentage of HPV 16 E_6/E_7 in cervical cancerswas 67.6% (23/34). This result showed that HPV 16 may play an importantrole in development and progression of cervical cancers, and use of the PCRtechnique for selective detecting the transforming genes(E_6/E_7) of HPV16 maybe helpful with research of cervical carcinogenesis and early finding of cervicalcancer patients.
出处
《中国病毒学》
CSCD
1992年第1期59-62,共4页
Virologica Sinica
关键词
人乳头瘤病毒
宫颈癌
聚合酶链反应
Human Papillomavirus
Transforming genes
Cervical cancers
Polymerase chain reaction
