摘要
目的 通过全国流行病学调查 ,探讨血清性激素水平变化与衰老的关系。方法 以健康自愿者 2 2 5 3例为研究对象 ,应用放射免疫法测定性激素 ;全自动生化仪测定血脂谱、血糖 (GLU)及载脂蛋白 (apo) ;用流式细胞仪测定T细胞亚群 ;采用硫代巴比妥酸法和Oyanagui的亚硝酸盐形成法分别测定血清丙二醛 (MDA)和超氧化物歧化酶 (SOD)。结果 老年男性随增龄促卵泡激素 (FSH)、黄体生成素 (LH )血清浓度均显著增加 ;睾酮 (T)、游离睾酮 (FT)随增龄明显下降 ,17β 雌二醇 (17β E2 )则相反 ,E2 /T比值随增龄明显升高。垂体泌乳素 (PRL)随增龄有所升高、孕酮 (P)变化不明显。血清胆固醇 (TC)随增龄升高 ,甘油三酯 (TG)无明显变化 ;与年轻组比 ,老年组高密度脂蛋白胆固醇 (HDL C)、HDL C/TC显著降低 ,同时低密度脂蛋白胆固醇 (LDL C)显著升高 ,HDL C/LDL C随增龄有所降低 ;apoA1和apoB均随增龄显著升高 ,apoA1/apoB比值则降低 ;GLU各年龄段无明显变化。与年轻组相比老年组CD3+ ,CD4 + 各组均明显下降 ;而CD8+ 随增龄明显升高 ,CD4 + /CD8+ 比值随增龄显著降低。与年轻组相比 ,老年组MDA明显升高 ,SOD却显著降低。通过偏相关分析 (双侧检验 ,控制BMI、FSH、LH、PRL)显示男性TC、LDL C、apoA1、apoB、CD8+ 。
Objectives To investigate the correlation between sex hormones and aging. Methods Through epidemiological investigation in our country, the levels of sex hormones were measured by radio-immunity method; lipid profile; glucose and apolipoprotein by automatic biochemic analytical instrument; T cell subsets by flow cytometer; and MDA; SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men, the serum levels of follicle stimulating hormone(FSH)?luteinizing hormone(LH) increased significantly with aging, but serum prolactin(PRL) and progesterone(P) remained unchanged in all life. Both testrone(T) and free testrone (FT) all decreased greatly with aging, but 17β-estradiol (17β-E2) was reverse; E2 was negatively correlated with T and E2/T increased with aging. The level of serum total cholesterol(TC) increased with aging, but triglycerides(TG) remain unchanged; compared with young group, high-density lipoproteir cholesterol (HDL-C), HDL-C/TC of other groups decreased significantly, but low-density lipoprotein cholesterol(LDL-C) changed inversely, HDL-C/LDL-C reduced slightly with aging and showed no difference between groups. Apolipoprotein A1(apoA1) and apoB all enhanced greatly with aging; meanwhile the ratio of apoA1/apoB decreased. The concentration of serum glucose (GLU) was unchanged in all life. To compare with those in young group, CD3 +, CD4 + in other groups reduced greatly, CD4 + remained unchanged. Meanwhile, CD8 + increased significantly with aging. Compared with the young group, serum malondialdehyde(MDA) value of the old ones increased obviously, but the activity of superoxide dismutase(SOD) was reverse. By partial correlation analysis (controlling BMI, FSH, LH and PRL), TC?LDL-C?apoA1?apoB?CD8 +?MDA of men all presented a positive correlation with E2/T respectively, their correlation coefficients(γ) were 0.262?0.136?0.532?0.379?0.394?0.234(P<0.001);HDL-C?HDL-C/TC?HDL-C/LDL-C?CD3 +?CD4 +/CD8 +?SOD showed a negatively correlation with E2/T respectively, γ equaled -0.563? -0.332?-0.654? -0.153 0? -0.414 0?-0.236(P<0.001). In women, the serum concentrations of FSH?LH increased significantly after menopause; PRL increased little with aging. Compared with young group, E2 and P in postmenopausal groups reduced obviously, E2/P revealed significant reduce with aging. T enhanced significantly after menopause, but nor did FT. E2, P and the ratio of E2/P were negatively correlated with age respectively by bivariate correlation analysis, and a positive relation between T and age. After 70 years old, the level of TC increased obviously, so did that of TG after menopause; HDL decreased with aging, but LDL increased after 70, with the result that the ratios of HDL-C/TC and HDL-C/LDL-C all reduced with aging, apoA1 decreased gently after 70, but apoB increased significantly after menopause, correspondingly, the ratio of apoA1/apoB declined obviously. The concentration of GLU increased with aging. CD3 + and CD4 + didn't change until 60, but reduced after 60. Compared with the young groups, CD8 + remained unchanged, CD4 +/CD8 + reduced greatly with aging, CD4 + and CD8 + presented a negatively correlation with age respectively. The value of MDA in serum of women increased notly after 70 years old, but SOD activity already decreased significantly from 60. By partial correlation analysis (controlling BMI, FSH, LH and PRL),HDL-C? CD4 +?CD4 +/CD8 + showed a certain correlation with E2/P respectively;γ were 0.245?0.157?0.154 (P<0.05); TG?LDL?apoB?apoA1/apoB?SOD presented a negatively correlation with E2/P respectively,γ were 0.452?0.236?0.321?0.135?0.156?0.154?0.426 (P<0.05). Conclusions The Disequilibrium of sex hormones had correlations with lipid profile, cellular immunological function and lipid peroxides and oxidation system; these suggested sex hormones took an important role in the process of aging.
出处
《岭南心血管病杂志》
2004年第1期10-16,共7页
South China Journal of Cardiovascular Diseases
基金
国家重点基础研究发展规划 [" 973"](G2 0 0 0 5 70 0 8)
军队重点科学基金 (No :0 1Z0 42 )
国家教育部高等学校骨干教师资助计划的资金
关键词
性激素
血脂
细胞免疫功能
氧化系统
抗氧化系统
衰老
超氧化物歧化酶
丙二醛
Aged man
Sex hormones
Lipid profile
Apolipoprotein
T lymphocyte subpopulations
Malondialdehyde
Superoxide dismutase
Epidemiological study
