摘要
采用游离肝细胞培养技术,用非极性毛细管气相色谱法测定大白鼠肝细胞中CCl_4并分离其氟代甲烷类代谢物。方法线性范围宽(0.1mmol/L～0.01μmol/L),灵敏度高(最低检测浓度为5×10^(-3)nmol/L)。结果还提示,CCl_4在进入肝细胞后,被分解成CHCl_3、CH_2Cl_2等代谢产物;CHCl_3、CH_2Cl_2可能为CCl_4致肝损伤的除脂质过氧化外的又一条途径。
The hepatocyte primary culture techniques and Non Polar Capillary Gas Chromatography were used to determine the amount of CCl_4 and its metabolites in hepatocyte, [The linear limit of this method was wide (0.1 mmol/L～0.01 μmol/L) and its sensitivity was high(the minimum concentration detected was 5×10^(-3) nmol/L). The result also showed that CCl_4 was transformed into CHCl_3 and CH_2Cl_2 after it had entered into hepatocytes. It is suggested that CHCl_3 and CH_2Cl_2 may be a kind of channel for eliciting liver damage other than lipid peroxidation.
出处
《职业医学》
1992年第5期258-260,共3页
China Occupational Medicine
基金
国家"七五"医学重点科技攻关项目
关键词
四氯化碳
肝细胞
代谢物
分离
测定
CCl_4,hepatocyte,Non Polar Capillary Gas Chromatography,CHCl_3,CH_2Cl_2
