摘要
珠子参(Panax japonicus C.A.Meyer var. major(Burk)Wu et Feng)根茎用盐溶液提取,透析,两次离子交换层析,凝胶过滤,分离到两个蛋白质(ZP-1,ZP-2),其中,ZP-2能明显地抑制绿色木霉、麦类赤霉菌丝的生长。经SDS-PAGE及Sephadex G-75过滤,测得ZP-2的分子量分别为55和66kD,是由分子量非常相近的两个亚基组成。PAS反应表明ZP-2是一个糖蛋白,经GLC分析,中性糖含量为28.91％,由葡萄糖、甘露糖、岩藻糖、木糖、鼠李糖和半乳糖组成;糖醛酸含量经Bitter的方法测得为22.76％。用OPA柱前衍生氨基酸高压液相色谱法测定ZP-2的部分氨基酸组成,结果表明此蛋白质高含酸性氨基酸。ZP-2对人红血细胞无凝集活性,也无几丁酶和β-1,3-葡聚糖酶活性。
Two proteins named ZP-1 and ZP-2 in rhizome of Panaxjaponicus var. major were isolated by the procedure including saline solution extraction, dialysis, ion exchange chromatography, and gel filtration. On PSA media, ZP-2 inhibited obviously the growth of mycelia of Trichodema viride and Fusarium graminearum. The protein contained two subunits (MW. 27 and 28kD), and its molecular weights were 55 and 66kD respectively determined by SDS-PAGE and Sephadex G-75 filtration. By PAS reaction, it was shown that ZP-2 was a glycoprotein, which contained glucose, mannose, fucose, xylose, galactose, rhamnose, and uronic acids. The contents of neutral sugars and uronic acids were 28.91% and 22.76% respectively. The protein was rich in Asp and Glu, but had no activity of hemagglutination, chitinase, and β-1, 3-glucanase.
出处
《云南植物研究》
CSCD
1992年第4期430-436,共7页
Acta Botanica Yunnanica
关键词
珠子参
糖蛋白
抗真菌蛋白质
Panax japonicus var. major
Glycoprotein
Antifungal protein
