摘要
用100、500、1000ppm-浓度的5406细胞分裂素和稀土分别诱导群众杨的离体枝条,3d后进行接种。实验证明,用“5406”发病率降低了63.3%~70%,病情指数降低了51.5~65.2;用稀土发病率降低了60%~70%,病情指数降低了29.1~73.9。接种后的群众杨皮部SOD活性在72~96h内达到最高峰,以后逐渐下降,并趋于稳定;呼吸强度48h达到最大值,96h明显减弱,并基本稳定。发病后,皮部总蛋白含量增加1.7~4.4倍(对照总蛋白含量仅增加0.9倍),增加的量随诱导剂的浓度增大而增多。
The branches of Populus X xiaozhuanica cv. ‘popularis ’are induced
byrare-earth and Streptomyces jingyanesis 5406( three concentrations of 1 00,500 and 1
000ppm in both inducers),then inoculated with Dothiorella gregaria. After challenged
inocula-tion,the incidence of disease and disease index dropped with a reduction of 60%~
70%and29.1~73.9 for the formor and 63.3%~70%and 51,5%~65.2%for the latter
respective-ly. The mechanism of resistance to canker disease is a series of physiological
changes takingplace in the bark of branches after induction. The activity of superoxide reached
the maxi-mum after 72~96 hours and then dropped gradually and tended to be stable,the
activity ofSOD in the induced branches are higher than that in the control ones;the respiration
came tothe maximum after 48 hours and got weak after 96 hours when disease is going on the
totalprotein of induced branches increased by 2.7~5.4 times while that of the control only
1.9times,The changes above are related to the concentration of inducers with the larger
theconcertration the better the effect.
出处
《林业科学研究》
CSCD
北大核心
1995年第1期78-81,共4页
Forest Research
关键词
杨树
溃疡病
诱导
抗性育种
抗病性
Populus X xiaozhuanica cv.
‘popularis’,poplar canker disease ,inducedresistance, resistance breeding