摘要
目的 :建立稳定表达人自身免疫调节因子 ( autoimmune regulator,AIRE)的细胞株。方法 :用 Fu GENE6转染试剂将含 AIRE c DNA的真核表达载体转染 He La细胞 ,经 G41 8筛选阳性克隆。继续培养 4周 ,用荧光显微镜观察、流式细胞仪 ( FACS)检测和免疫印迹法检测 AIRE基因在 He La细胞中的稳定表达情况。结果 :经荧光显微镜、流式细胞仪及免疫印迹法检测 ,证实 AIRE基因得到了稳定表达。结论 :在 Fu GENE6转染试剂的介导下 ,AIRE基因在 He
Objective: To establish HeLa cell line with stable expression of human autoimmune regulator. Methods: Eukaryotic expression vector plasmid containing AIRE cDNA was transfected into HeLa cells with FuGENE6 Transfection Reagent. The positive cell clones had been obtained after selected by G418. The stable transfection and expression of AIRE in HeLa cells were determined by the fluorescence microscope, FACS and Western blotting. Results: Stable expression of AIRE was confirmed by fluorescence microscope, FACS and western blotting analysis. Conclusion: With FuGENE6 transfection reagent, AIRE gene was stably expressed in HeLa cells that would be helpful for further study on AIRE gene.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第2期155-157,共3页
Journal of Jilin University：Medicine Edition
基金
吉林大学创新基金 (2 0 0 2 )
