摘要
目的 :探讨恶性肿瘤MDR1基因治疗过程中 ,MDR1基因在体内的表达。方法 :骨髓造血细胞转染MDR1基因后 ,程序性移植入预先照射亚致死剂量60 Co -γ射线的荷瘤小鼠 ,取骨髓造血细胞、肿瘤实体做P -糖蛋白免疫组化并做RT -PCR以观察MDRI基因的表达情况。结果 :各剂量组MDRI基因转染后随时间骨髓造血细胞P -糖蛋白表达逐渐增加 ,各剂量组之间无明显差异。P -糖蛋白表达化疗剂量较大组明显高于化疗剂量较小组。结论 :MDRI基因转染在骨髓造血细胞有较高表达 ,对肿瘤实体、肝脏、肾脏P -糖蛋白表达无影响。
Objective:The study was to improve the protection effect of the bone marrow during the chemotherapy of earlier hepatocarcinoma Balb/C mice,to observe the expression of P-gp in tumor tissues and important organs and to examine the influence of MDR1 gene on Balb/C mouse. Methods:MDR1 gene was transferred into hematopoietic cells of murine bone marrow by the mediation of retrovirus vecter and transplanted into the Balb/c mouse with hepatocarcinoma exposure with 60 Co-γ ray in advance.The function and the expression of MDR1 gene in hematopoietic cells,and tumor tissue was detected by RT-PCR method and IC method in vitro.Results:There was no P-gp expression in the two control groups,but that of the different dose experiment group was higher than that of control groups obviously ( P <0.01).The P-gp expression of every experiment groups rose following the high-dose chemotherapy time.The P-gp expression was higher than the following chemotherapy dose of every group.Conclusion:There were higher P-gp expression in hematopoietic cells with MDR1 gene transplanted into the body of the bone in the Balb/C mice with hepatocarcinoma.
出处
《重庆医科大学学报》
CAS
CSCD
2003年第3期279-283,共5页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目 (No .3 0 0 70 781)
