摘要
目的 探讨内皮细胞生长因子 (Endothelialcellgrowthfactor ,ECGF)诱导的血管内皮细胞 (Vascularendothelialcell ,VEC)增殖过程中FK5 0 6结合蛋白 12 (FK5 0 6bindingprotein 12 ,FKBP12 )表达的变化 ,为VEC的鉴定提供新的方法。方法 培养大鼠VEC并传代。倒置显微镜下观察其在ECGF作用下的增殖情况 ,检测Ⅷ因子相关抗原、FKBP12和不同时期FKBP12mRNA水平的表达。结果 VEC表达FKBP12 ;VEC传代后第 4天FKBP12mRNA表达水平达到最高 ,6～ 8d下降。结论 FKBP12既可作为VEC的标志物 。
Objective To study the changes of expression of FK506 binding protein 12 (FKBP12) in vascular endothelial cell (VEC) proliferation induced by endothelial cell growth factor (ECGF) and to provide a new way for the identification of VEC. Methods Rat vascular endothelial cells were cultured and passaged. After the induction of ECGF, the proliferation of VEC was observed under the invert microscope; factor Ⅷ related antigen and FKBP12 were detected by immunocytochemistry; expression levels of FKBP12 mRNA in different culture periods were determined by semi quantitative RT PCR. Results The expressions of FKBP12 were observed in cultured endothelial cells. The expression level of FKBP12 mRNA reached a peak at 4 d after passage, and then decreased at 6～8 d. Conclusion The expression of FKBP12 may be a new maker in cultured endothelial cells, and can reflect the proliferation of cultured endothelial cells as well.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第24期2205-2207,共3页
Journal of Third Military Medical University
