摘要
目的 研究转化生长因子 (TGF- β1)、牙本质非胶原蛋白 (d NCPs)及 TGF- β1/ d NCPs复合物对组织工程牙髓形成的促进作用。 方法 使用 型胶原及牙本质粉构建牙髓细胞的三维培养模型 ,按不同分组分别在组织工程牙髓中加入 TGF- β1、d NCPs及 TGF- β1/ d NCPs复合物 ;对照组中不加入以上物质。培养 3、6和 14天 ,在不同时间点行 HE染色 ,观察细胞的形态变化。牙本质涎蛋白 (DSP)免疫组织化学观察牙髓细胞向成牙本质细胞的诱导。 结果 胶原可形成凝胶网状结构 ,牙髓细胞的生长状况与体内牙髓细胞相似。加入 TGF- β1和 d NCPs后 ,培养第 6天部分牙髓细胞开始出现成牙本质样细胞的一些特性 ,出现单侧细胞突起 ;培养第 14天 ,牙本质粉区域附近细胞呈高柱状平行排列 ,形成类似体内的牙本质牙髓复合物。免疫组织化学染色可见部分细胞出现 DSP的表达 ,其中 TGF- β1组阳性细胞最多 ,复合物组次之 ,d NCPs组最少 ;而对照组未发现 DSP的表达。 结论 TGF- β1和 d NCPs可不同程度地刺激牙髓细胞向成牙本质样细胞转化 ,促进组织工程牙髓的形成。
Objective To study the influence of transforming growth factor-β1(TGF-β1), dentin non-collagen proteins(dNCPs) and their complex on tissue engineering pulp system. Methods Collagen I and dentin powder were used to construct the system of pulp cells in 3-dimensional culture, dentin powder was added in the gel. The tissue engineering pulp were divided TGF-β1 group, dNCPs group, TGF-β1/dNCPs group and control group.After 3, 6 and 14 days, the appearance and the differentiation of pulp cells were observed by HE staining and immunohistochemical staining respectively. Results Collagen I could form netted collagen gel construction. Growing condition of pulp cells in gel was similar to that of pulp cells in vivo. After the TGF-β1 and dNCPs were added, the pulp cells had some characteristics of odontoblasts and had unilateral cell process after culture 6 days. Pulp cells arranged with parallel columnar and form dentin-pulp-like complex after 14 days. Immunohistochemical staining showed dentin salivary protein(DSP) began to express in some cells. The number of positive cell was most in the TGF-β1 group. No positive cells were detected in the control group. Conclusion The transforming growth factor-β1 and non-collagen proteins can stimulate the pulp cells to transform into odontoblasts to some extent, which promote the formation of tissue engineering pulp.
出处
《中国修复重建外科杂志》
CAS
CSCD
2004年第2期115-118,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家高技术研究发展计划 (863)资助项目(2 0 0 2 AA0 50 4 1 )~~
