摘要
目的研究新型盖髓材料磷酸钙/硅酸钙/泡铋矿复合水门汀(Calcium phosphate-calcium silicate-bismuth compound cement,CPCSBi)诱导牙髓细胞分化的作用。方法分别采用CPCSBi、氢氧化钙(CH)饱和溶液和10%EDTA溶液,从人牙本质粉末中提取可溶性牙本质基质(DDMCs),通过半定量RT-PCR方法,分析不同材料所提取DDMCs对人牙髓细胞的涎磷蛋白(DSPP)、骨钙素(OCN)、转化生长因子-β1(TGF-β1)基因表达的影响。结果不同材料提取的DDMCs都能提高体外培养人牙髓细胞DSPP、OCN和TGF-β1基因的表达,其中以CPCSBi溶液提取DDMCs的促进作用最明显。结论 CPCSBi对牙髓细胞分化具有促进作用。
Objective The aim of this study was to investigate the effects of new calcium phosphate/cal- cium silicate/bismutite （CPCSBi） cement on differentiation of human dental pulp cells. Methods Dissoluble dentin matrix components （DDMCs） were extracted from powdered sound, human dentine samples by either CPC- SBi saturated solution, Ca（OH）2 saturated solution, or 10% EDTA, over a 14-day period. The effects of DDMCs extracts on dentin sialophosphoprotein （DSPP）, osteocalcin （OCN） and TGF- 13 1 gene expression in the dental pulp cells were analysed using semi-quantitative RT-PCR following 24 h of exposure. Results All the DDMCs extracts induced DSPP, OCN and TGF- β1 gene expression in dental pulp cells. While the DDMCs extracted from dentin powder by CPCSBi demonstrated highest inducing effect on expression of DSPP and OCN in human pulp cells. Conclusion These results suggest that CPCSBi plays an important role in the differentiation of dental pulp cells to odontoblast like cells.
出处
《口腔材料器械杂志》
2013年第3期122-125,140,共5页
Chinese Journal of Dental Materials and Devices
基金
上海交通大学医学院博士创新基金(BXJ0731)
关键词
盖髓材料磷酸钙
硅酸钙
泡铋矿人牙髓细胞分化
Pulp-capping agent Calcium phosphate/calcium silicate/bismutite Human dental pulp cellDifferentiation
