摘要
目的 探讨甲基莲心碱 (Neferine ,Nef)对骨肉瘤细胞 (Saos 2cells)的体外化疗增敏作用。方法 以人骨肉瘤细胞株 (Saos 2cells)为研究对象 ,MTT法测定药物的细胞生长抑制率 ,流式细胞仪 (FlowCytometry ,FCM)检测细胞凋亡。 结果 Nef(5 ,10 μmol·L-1)分别为与阿霉素 (ADM) ,5 氟尿嘧啶 (5 Fu) ,顺铂 (CDDP) ,鬼臼乙叉苷 (VP16 )合用 ,可增加它对Saos 2细胞的生长抑制率 ,q >1。Nef(10 μmol·L-1)可增强不同浓度ADM诱导Saos 2细胞凋亡。结论 Nef对Saos 2细胞有化疗增敏作用。
OBJECTIVE To study the enhancement of cytotoxicity of anticancer drugs in vitro by neferine(Nef) in Saos-2 cells. METHOD Cytotoxicity assay was tested by MTT method, modulation of adriamycin(ADM)-induced apoptosis by Nef in Saos-2 cells was detected by Flow cytometer (FCM). RESULTS The inhibitory rates (IR) of Nef (5, 10μmol ·L -1)combined with ADM ,5-Fu,CDDP and VP16 were higher than those of these drugs used alone ,and q values were more than 1.Nef (10μ mol·L -1)promoted apoptosis induced by ADM in Saos-2 cells.CONCLUSION:Nef increases the sensitivity of Soas-2 cell to anticancer drugs.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2004年第1期4-5,共2页
Chinese Journal of Modern Applied Pharmacy
基金
湖南省教委科研基金资助项目 (No:9615 5 )
