摘要
目的:研究淫羊藿次苷Ⅱ,知母皂苷AⅢ,甲基莲心碱,红景天苷对PANC-1移植瘤的作用。方法:建立PANC-1皮下移植瘤模型,成瘤后随机分组,治疗组给予中药单体腹腔注射,23 d取瘤称重,部分组织中性福尔马林固定做免疫组化,其余组织液氮速冻,然后转移至-80℃冰箱保存做基因蛋白分析。免疫组化Ki67观察肿瘤增殖情况,RT-PCR检测瘤组织VEGF mRNA表达情况,Western blotting检测瘤组织VEGF蛋白表达。结果:与模型组比较,知母皂苷AⅢ(1.0 mg·kg-1)能抑制肿瘤生长(P<0.05),能降低VEGF mRNA(P<0.05)和VEGF蛋白表达(P<0.05),活化Caspase-3蛋白。淫羊藿次苷Ⅱ,甲基莲心碱,红景天苷的抗肿瘤作用不显著。结论:知母皂苷AⅢ其抗肿瘤作用与抑制VEGF及促进肿瘤凋亡有关。
Objective： The antitumor effects of icarisid Ⅱ , timosaponin A-Ⅲ, neferine and salidroside were studied in PANC- 1 xenograft tumor. Method： To establish of the nude mice xenograft tumor model, PANC-1 cells were injected. When the tumor major diameter was reached 3-5 mm, the treatment was initiated. The mice were randomized into vehicle control and treatment groups of six animals per each. Chinese medicine monomer was injected intraperitoneally every day. In 23th day, mice were killed once a day, tumor tissue were isolated and weighed and divided into two parts. One part was fixed with formaldehyde for tissue section and immuno- histochemistry, the another of tissue was frozen in liquid nitrogen then in - 80 ℃ refrigerator for gene and protein expression analysis. Result： In PANC-1 tumor xenograft experiment, compared with model group, timosaponin A-Ⅲ （1.0 mg · kg^- 1 ） exerted significant inhibito- ry effects on tumor growth. Timosaponin A-m suppressed mRNA expressions of VEGF （P 〈0. 05）, reduced protein expressions of VEGF （P 〈 0. 05）, activated Caspase-3 protein. Icarisid Ⅱ, neferine and salidroside had not an excelled antitumor effect. Conclusion： Timosaponin A-Ⅲ exerted an excelled antitumor effect. Tile antitumor mechanisms include anti-angiogenesis, apoptosis promotion.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第2期245-248,共4页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09311-003)
上海市教委高校一氧化氮与炎症医学E研究院计划项目(E-04010)
关键词
中药单体
血管内皮生长因子
凋亡
Chinese medicine monomer
vascular endothelial growth factor
apoptosis
