摘要
目的:观察老年人血清中脑自身抗体改变。方法:用3月龄Wistar大白鼠脑组织冷冻切片作固定抗原,用FITC-山羊抗人多价免疫球蛋白与人血清标本中抗体结合,在荧光显微镜下判断人血清脑自身反应抗体阳性反应。结果:无神经系统疾病老年人血清脑自身抗体阳性率(++:36.8%;+:52.6%)均显著高于年轻人(++:0;+:10.5%;均为P<0.01)。结论:随着年龄增长,在即使无神经系统疾病老年人血清中脑自身反应抗体也明显增高。提示脑自身反应抗体对大脑功能的影响应引起注意。
AIM:To observe the changes of serum brain-reactive antibodies(BRA) in the elders. METHODS:The slices of frozen brain obtained from 3-month Wistar rats were used as antibody fixation.Positive brain-reactive antibodies were observed under fluorescence microscope following FITC conjugated goat anti-human polyvalent immunoglobulins binding human serum antibodies. RESULTS:The positive rates of serum BRA in the elders without CNS diseases were 36.8%(++) and 52.6%(+) respectively,both higher than those of the young(++:0;+:10.5%;both P< 0.01). CONCLUSION:BRA is increased with aging in the blood serum of the elders,even in those without CNS diseases.It is indicated that we should attach importance to the effects of autoantibodies to brain function.
出处
《中国临床康复》
CSCD
2004年第7期1284-1285,T003,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家计委攻关项目(85-722-35-02)~~
