摘要
目的:研究视网膜光损伤与视细胞凋亡的相互关系,以探讨视网膜光损伤的发生发展机制。方法:所有SD大鼠经循环光环境适应7d,实验前暗适应36h,分别于光照3,6,9,12,15和18h,灌流固定,摘除眼球。光镜标本在常规脱水、透明、石蜡包埋切片后,行苏木精-伊红(HE)、TUNEL法染色,光镜观察;电镜标本在树脂包埋、超薄切片、醋酸-柠檬酸铅双重染色后,透射电镜观察;应用CIAS-1000图像分析系统定量检测外核层面积和视细胞凋亡指数,所得数据作统计学分析。结果:可见视网膜出现光损伤和视细胞凋亡现象,随着光照时间的延长,视网膜光损伤逐渐加重,视细胞凋亡逐渐增多。在外核层,透射电镜观察见核染色质浓集,而无炎性反应。外核层面积和视细胞凋亡指数作相关性分析有显著意义。结论:视细胞凋亡是视网膜光损伤的重要机制;光损伤启动了视细胞凋亡的发生,外核层细胞核的丢失是视细胞凋亡的结果;视网膜光损伤与视细胞凋亡有着密不可分的联系。
AIM:To observe the relationship between retinal photic injury and receptor cell apoptosis,exploring the development mechanism of retinal photic injury. METHODS:SD rats were maintained under a cyclic light/dark environment for 7 days and then kept in darkness for 36 hours before experiment.The rats were sacrificed after continuous intense white light exposure for 3,6,9,12,15,and 18 hours.Light microscopy samples were enucleated and fixed after perfusion,then processed and embedded in paraffin using routine procedures.All sections for examination under light microscopy were made by Haematine Eosin(HE) and TUNEL staining.Sections prepared for electron microscopy were embedded in colophony,then they were sliced into ultrathin sections and dyed by acetate citric lead.The outer nuclear layer area and cell apoptosis index were quantified using the CIAS 1 000 cell photo analysis system, and the data were statistically analyzed. RESULTS:Photic injury and receptor cell apoptosis were found in the retina.Photic injury in the retina worsened and optic cell apoptosis increased as time of light exposure increased.Condensation of chromatin did not reveal any inflamatory response under electron microscopy.The relationship between outer nuclear layer area(OA) and the apoptosis index(AI)was compared using relative analysis.There were significant differences between them. CONCLUSION:Apoptosis is an important mechanism in light induced retinal damage.Light triggers the apoptosis of photoreceptor cells.The loss of cells in OA is the result of apoptosis. Photic injury closely correlated to receptor cell apoptosis.
出处
《中国临床康复》
CSCD
2004年第5期998-1000,F003,共4页
Chinese Journal of Clinical Rehabilitation
基金
辽宁省教育厅科技基金(991721149)~~
