摘要
用薄层聚丙烯酰胺凝胶等电聚焦电泳分析了上海地区202例汉族胃癌病人及202例正常对照组的运铁聋白(Tf)和α_1-抗胰蛋白酶(α_1-AT)亚型的分布,发现胃癌组Tfc_1c_1纯合子频率(0.3713)和Tfc_1基因频率(0.5718)显著低于对照组(分别为0.5149和0.6782),均为p<0.01,胃癌组Tfc_2c_2纯合子频率(0.2228)和Tfc_2基因频率(0.4019)显著高于对照组(分别为0.1436,p<0.05和0.2970,p<0.01),胃癌组和对照组的α_1-抗胰蛋白酶亚型分布无显著差异。用薄层聚丙烯酰胺等电聚焦结合免疫固定分析了上海地区200例汉族胃癌病人和200例正常对照组的组特异性成分(Gc)亚型分布,发现胃癌组Go1F表型频率(0.22)和Gc1F基因频率(0.4375)均显著高于正常对照组(分别为0.14和0.3600,均为p<0.05)。
The genetic polymophisms of transferrin (Tf) and drAntitrypsin (α1-AT) in 202 gastric cancer patients and 202 controls in Shanghai Hans were analyzed by isoelectric focusing. In transferrin, the phenotype frequency of C1C1 and gene frequencies of c1 and c2 were 0.3713, 0.5718 and 0.4109 respectively in gastric cancer patients, and 0.5149, 0.6782 and 0.2970 separately in controls. The frequencies of the c1c1 phenotype and the C1 gene increased significantly (p<0.01) among the controls, while the frequency of the C2 gene greatly increased (p<0.01) among che patients. The frequencies of the C2C2 in gastric cancer patients and controls were 0.2228 and 0.1436 respectively, showing a significant difference between them (p<0.05).The genetic polymorphisms of group-specific component (Gc) in 200 gastric cancer patients and 200 controls in Shanghai Hans were studied with isoelectric focusing followed by immunofi-xation. The GclFlF phenotype frequency and IFgent; frequency were 0.22 and 0.4375 in gastric cancer patients, and 0.14 and 0.3600 in controls, respectively. The frequencies of the GclFlF phenotype and the IF gene were significantly increased (p<0.05) among the patients.In α1-Antitrypsin, there was no significant difference in phenotype frequencies and gene frequencies between the patients and controls.The data reported here indicate that the gastric cancer is associated positively with TfC2 and GclF, and negatively with TfC1. These facts support the notion that cancerogenesis is a multi-step process controlled by multifactorial inheritance.
关键词
运铁蛋白
胃肿瘤
抗胰蛋白酶
GC
Gastric Cancer, Transferrih(Tf), Group-Specific Component(Gc), α_1-An-titrypsin (cu-AT), Polyacrylamide gel electrophoresis (PAGE), Isoelectric focusing (IEF)
