摘要
目的 了解经肛门途径清洁灌肠及经口服途径全肠道灌洗对幼龄兔肠黏膜屏障功能及细菌移位的影响 ,以期选择更为安全的方法 ;并对灌肠后细菌及内毒素移位的机制进行探讨。方法 选用幼龄兔 30只 ,随机分成 3组 ,每组 10只 ,分别为经肛灌肠组 (A) ,经口全肠道灌洗组 (B)及空白对照组 (C)。依次取肠系膜淋巴结 (MLN)、肝、脾、肺匀浆 ,并抽取门静脉血液、胆汁 ,分别行细菌培养及药敏实验 ,并行血浆内毒素测定。结果 口服清肠组及灌肠组与对照组三者之间差异均有显著性 (P <0 0 5 )。结论 灌肠组和口服清肠液组 ,都可明显地引起肠道细菌移位 。
Objective To probe into the relationship and mechanism between different enteroclysis and intestinal bacterial translocation in infancy rabbit in order to choose better way. Methods Thirty infancy rabbits were randomized into three groups, each received different enteroclysis. The results of bacterial cultivation, clonal counting, and the level of plasma endotoxin in different tissues and organs were compared. Results The total translocational rates were 13 3%(enteroclysis group) , 8 3%(peroral group), 1 6%(control group). The level of plasma endotoxin for the 3 groups were (425±83)×10 -6 EU/L,(30±6)×10 -6 EU/L and (50±9)×10 -6 EU/L respectively. The difference between different groups was statistically significant ( p< 0 05). Conclusion Bacterial translocation can be caused by both kinds of enteroclysis, and the peroral lovation is better than the enterclysis.
出处
《安徽医科大学学报》
CAS
2004年第1期40-42,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽省卫生厅资助科研课题 (编号 :1998 162 )
