摘要
目的:通过研究不同人群中新发现的高血压相关基因HRG-1基因多态性,以揭示高血压相关基因多态性与高血压的关系。方法:选取74例正常对照,其中男38例,女36例,平均年龄(54.15±7.77)岁。原发性高血压患者51例,其中男27例,女24例,平均年龄(57.25±7.97)岁;高血压家族患者20例,其中女11例,男9例,平均年龄(37.97±21.93)岁。取外周静脉血并提取DNA,设计相应的引物,聚合酶链反应(PCR)扩增法获得相应的产物,应用DNA测序法直接得到PCR产物的核苷酸序列后比较不同人群的相同片段的碱基组成及其特征。结果:肌酐和尿素氮在高血压组明显高于正常血压组(F=17.25,2.99;P=0.000,0.060),而高血压组和高血压家族组的收缩压和舒张压均明显高于正常血压组(F=19.25,6.68;P=0.000,0.002)HRG-1基因第12位内含子的21位点(1q82153)的C-G单核苷酸变异在高血压组与正常人之间存在着明显的差别(P<0.05),高血压家族组与正常人也存在明显差别(P<0.01)。C/G等位基因在不同人群中存在着类似的差别。且在人群中以CC基因型分布较为常见(P<0.01)。结论:肾功能的指标(肌酐和尿素氮)可能可以较早地反映高血压的进展和靶器官损害状况。HRG-1基因第12位内含子的21位点,即1q82153的C-G单核苷酸变异及基因频率在中国汉族不同的人群中存在着明显的?
AIM:To investigate the single nucleotide polymorphism (SNP) of the novel hypertension related gene 1(HRG 1) and to reveal the relationship between HRG 1 SNP and hypertension. METHODS:Seventy four normal people(38 males,36 females) with a mean age of(54.15± 7.77) years old, 51 patients with essential hypertension(27 males,24 females) with a mean age of(57.25± 7.97) years old and 20 patients with hypertension of family history(9 males,11 females) with a mean age of(37.97± 21.93) years old were chosen in the study. The peripheral venous blood was drawn and the DNA was extracted,right primers were designed,and corresponding products were obtained by the method of polymerase chain reaction(PCR) amplification.The nucleic acid sequences of each PCR product were obtained directly by DNA sequencing, and the base conformation and character of different segments in different crowds were compared. RESULTS:The creatinine and blood uria nitrogen(BUN) in the hypertensive group were obviously higher than that in the normal control group(F=17.25,2.99;P=0.000,0.060),while the systolic pressure and diastolic pressure were obviously higher in the hypertensive group and hypertensive family history group than in the control group(F=19.25,6.68;P=0.000,0.002).There were significant differences in the C G SNP variation of 1q 82153 between the hypertensive group and the control group(P< 0.05),and the significant differences also existed between the hypertensive family history group and the control group(P< 0.01).There were similar differences in C/G alleles among different crowds,and the distribution of CC genotype was common. CONCLUSION:The renal function indices(creatinine and BUN) can reflect the progress of hypertention and damage state of target organ earlier. The 1q 82153 C G SNP variation and gene frequency of HRG 1 are obviously different among different crowds in the Chinese Han nationality,and the SNP is maybe an independent risk factor for hypertension.
出处
《中国临床康复》
CSCD
2004年第6期1060-1062,共3页
Chinese Journal of Clinical Rehabilitation
基金
北京心血管病研究实验室资助(H010910070113)~~
