摘要
为了研究一个新的人高血压相关基因HRG 1的编码蛋白在大肠杆菌中的表达及生物学活性 ,使用PCR的方法扩增人HRG 1基因ORF的编码序列 ,构建了原核表达质粒 ,并在大肠杆菌中诱导表达及进行体外活性鉴定。实验结果证明得到了具有生物学活性的人HRG 1原核表达蛋白。且人HRG 1原核表达蛋白具有与真核表达蛋白一致的生物学活性。
To study a novel gene related to hypertension encoded protein was expressed in E. Coli and its activity, the human HRG 1 generic open reading frame was obtained by PCR, and a prokaryotic expression system of human HRG 1 was constructed and biological activity were analyzed. The result showed that a prokaryotic expression protein of human HRG 1 was obtained, and the biological activity are agreeable to prokaryotic expression protein and eukaryocyte.
出处
《高技术通讯》
EI
CAS
CSCD
2000年第11期4-7,共4页
Chinese High Technology Letters
基金
863计划资助项目
