摘要
卡托普利先经衍生化形成具有紫外吸收的衍生物,然后用6％的高氯酸去蛋白,取上清液直接进样。以μBoundapak C_(18)(37～50μm)作为预处理柱(5cm×O.5cm,ID),对样品进行自动浓缩净化处理,预处理流动相为0.2％醋酸溶液。分析柱(15cm×O.5cm,ID)固定相为YWG-C_(13),10μm,流动相为乙晴-水-醋酸(35:65:0.4)。UV 260nm检测。血浆和尿样测定的线性范围分别为20～1000ng/ml和10～200μg/ml,血浆中最低检测浓度约为10ng/ml。方法的平均回收率分别为103.2％(血浆)和99.5％(尿样),日内及日间变异均小于10％。
A rapid and sensitive column-switching high performance liquid chromatographicprocedures was described for determination of captopril in plasma and urine, ρ-Bromophenacylbromide, a derivatizing reagent, was added to the plasma and urine samples to form an adduct whichshowed ultraviolet absorbing properties. After that, the urine sample was injected directively and forplasma the protein was removed with 6% of perchloride before injection. The column-switching sys-tem was equipped with a pre-column of 5 cm×0.5 em ID, packed with μBoundapak C_(18),37～50μm,and an analytical column of 15 cm×0. 5 cm ID, packed with YWG-C_(18),10μm. After washing outthe imputes from pre-column with 0.2% acetic acid the retained substances were eluted into analyticalcolumn with acetonitrile-water-acetic acid(35:65:0.4). Captopril was detected at 260 nm. Themethod was linear within 20～1000 rig/ml for plasma and 10～200 μg/ml for urine. The recovery av-eraged 103. 2% and 99.5% for plasma and urine, respectively. The coefficient variations were all le-ss than 10%.
出处
《药学学报》
CAS
CSCD
北大核心
1992年第8期613-617,共5页
Acta Pharmaceutica Sinica
关键词
卡托普利
高效液相色谱法
柱切换
Captopril
High performance liquid chromatography
Column switching
