摘要
应用聚丙烯酰胺凝胶电泳技术,对九孔鲍(Haliotisdiversicolorsupertexta)的养殖群体和杂色鲍(H.diversicolordiversicolor)的自然群体进行了10种等位酶的电泳检测和谱带遗传分析,在研究九孔鲍的18个等位酶位点和27个等位基因中,单态位点有Ldh -1、Adh -1、Sod-1、Sod-2、Sod-3、Est-1、Mdh -2、Me-1、Idh -1、Sdh-1、Sdh -2、Amy-2、Amy-3,在这些位点只有一个等位基因(Sdh -2除外);有5个位点是多态的,它们是Est-2、Est-3、Mdh-1、Aat-1、Amy-1(P0.99 标准),多态位点的百分数为27.78%,在这些位点有2～4个等位基因。在研究杂色鲍的18个等位酶位点和26个等位基因中,单态位点有Ldh -1、Adh -1、Sod-1、Sod -2、Sod -3、Est-1、Mdh-2、Idh -1、Sdh-1、Sdh -2、Amy-2、Amy-3,在这些位点只有一个等位基因;有6个位点是多态的,它们是Est-2、Est-3、Mdh-1、Me-1、Aat-1、Amy-1(P0.99标准),多态位点的百分数为33.33% ,在这些位点有2～4个等位基因。九孔鲍和杂色鲍在所有位点中共享大多数常见等位基因 ,因此在生化遗传上它们非常相似。本研究揭示了九孔鲍和杂色鲍群体等位酶位点及其等位基因带谱的变异式样 ,为九孔鲍和杂色鲍的遗传结构及遗传育种的研究提供了一批等位酶位点及其等位基因的参考图谱。
Biochemical genetics were investigated using the assay of vertical slab polyacrylamide gel electrophoresis in Haliotis diversicolor supertexta and Haliotis diversicolor diversicolor. Ten enzymes presumably encoded by 18 allozyme loci and 27 alleles were scored in H. diversicolor supertexta, the monomorphic loci were Ldh-1,Adh-1,Sod-1,Sod-2,Sod-3,Est-1,Mdh-2,Me-1,Idh-1,Sdh-1,Sdh-2,Amy-2,Amy-3(except Sdh-2 ); and 5 loci with 2～4 alleles were polymorphic, they were Est-2,Est-3,Mdh-1,Aat-1,Amy-1. The percentage of polymorphicloci(P0.99) is 27.78%. Ten enzymes presumably encoded by 18 allozyme loci and 26 alleles were scored in H. diversicolor diversicolor, the monomorphic loci were Ldh-1,Adh-1,Sod-1,Sod-2,Sod-3,Est-1,Mdh-2,Idh-1,Sdh-1,Sdh-2,Amy-2,Amy-3, and 6 loci with 2～4 alleles are polymorphic, they are Est-2,Est-3,Mdh-1,Me-1,Aat-1,Amy-1. The polymorphicloci(P0.99) percentage was 33.33%. H. diversicolor supertexta and H. diversicolor diversicolor shared most common alleles in all loci, they are biochemically genetically very similar. The bands represented loci and alleles of H. diversicolor supertexta and H. diversicolor diversicolor are showed in this paper. This paper shows the genetic diversity and structure of H. diversicolor supertexta and H. diversicolor diversicolor through comparative diagrams of allozymes.
出处
《海洋科学》
CAS
CSCD
北大核心
2004年第2期27-31,共5页
Marine Sciences
基金
国家自然科学基金资助项目(30231013)
福建省自然科学基金资助项目(B0110036)
关键词
九孔鲍
杂色鲍
等位酶
生化遗传
Haliotis diversicolor supertexta
Haliotis diversicolor diversicolos
allozyme
biochemical genetics
