摘要
应用高效液相色谱法对无环鸟苷的三种不同给药途径在家兔眼部分组织的移行浓度进行测定。实验分组选用3%眼膏(40mg)涂入结膜囊,1.5%注射液(0.1mL)注入球结膜下,0.5%注射液(5mg/kg)静注。按测定时点取角膜、房水、网脉络膜、血清做为试样,预处理后进行测定。选用Zorbax-ODS 色谱柱,流动相是0.0025M 庚烷磺酸钠和0.005M 醋酸钠混合液(pH6.5)。紫外检测波长254nm,检出界限波度为0.39μg/mL。此法简便、快速、灵敏度高,适用于眼科微量药动学的研究。
The intraocular penetration of Acyclovir(ACV)after topical and systemic administra-tion in rabbits was measured successfully by high performance ligid chromatography(HPLC).The method of administration was as follows:1.Spain on conjunctival sac(3%ointment 40mg).2.Subconjunctival injection(1.5% solution 0.1ml).3.Intravenous injec-tion in rabbit ear(0.5% solution 5mg/kg).After administration,the cornea,aqueoushumour,retinochoroid and serum were adopted as determined samples.The wave Lengthof UV detector was 254nm.The lowest detection limits of ACV was 0.39ug/ml.Because ofits convenience and sensitivity,the method is suitable for ophthalmic pharmacologicalresearch and clinical therapeutic monitoring.
出处
《眼科研究》
CSCD
1992年第4期221-224,共4页
Chinese Ophthalmic Research
关键词
高效液相色谱
无环鸟苷
角膜
high-performance ligid chromatography(HPLC)
acyclovir(ACV)
cornea
aqueous humour
