补阳还五汤药物血清对体外培养大鼠皮层神经元缺氧后p53和p21表达的影响被引量：31

Effect of Buyang Huanwu Decoction Drug Serum on Expression of p53 and p21 Genes in Cultured Rat's Cerebral Cortical Neuron after Hypoxia in Vitro

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摘要目的:探讨补阳还五汤对体外培养大鼠皮层神经元缺氧凋亡的作用,以及对神经元缺氧过程p53、p21基因表达的影响。方法:采用Daniel方法建立神经元缺氧凋亡模型,加入补阳还五汤药物血清,应用碘化丙啶(propidium iodide,PI)染色法经流式细胞仪检测神经元凋亡率,免疫组化法流式细胞仪检测p53和p21的表达。结果:补阳还五汤显著抑制缺氧导致的神经元凋亡,下调p53和p21基因的表达。结论:补阳还五汤对神经元缺氧凋亡有抑制作用,p53和p21基因表达下调是其机制之一。 Objective: To explore the effect of Buyang Huanwu decoction (BHD) drug serum on rat's in vitro cultured cerebral cortical neuron apoptosis induced by hypoxia, and on the expression of p53 and p21 genes in hypoxia process. Methods: The model of hypoxia neuron apoptosis was established adopting Daniel method and treated with BHD drug serum. The neuron apoptosis rate was determined by flow cytometry with propidium iodide staining, the p53 and p21 gene expression was tested by immunohistochemical method with flow cytometry. Results: BHD could significantly inhibit the neuron apoptosis induced by hypoxia and down-regulate the expressions of p53 and p21 genes. Conclusion: BHD shows inhibition on neuron hypoxia apoptosis and down-regulating of the p53 and p21 gene expression is one of its mechanisms.

作者曲宏达佟丽沈剑刚陈育尧刘晓伟

机构地区第一军医大学中医系香港大学内科学系物理科

出处《中国中西医结合杂志》 CAS CSCD 北大核心 2004年第2期133-135,共3页 Chinese Journal of Integrated Traditional and Western Medicine

基金国家自然基金资助项目(No.39970900) 广东省中医药管理局基金项目(No.100006)

关键词补阳还五汤药物血清体外培养皮层神经元缺氧 P53 p21 表达 Buyang Huanwu decoction, neuron, hypoxia, apoptosis, p53, p21

分类号 R285 [医药卫生—中药学]

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