摘要
目的 研究细胞增殖核抗原 (PCNA)及 DNA倍体水平的不同表达与结肠癌临床分期和预后之间的关系。方法 应用免疫组织化学方法及图像分析技术检测 65例结肠癌患者癌组织、癌旁正常粘膜的 PCNA标记指数和 DNA相对含量。结果 结肠癌组织中 PCNA标记指数和DNA相对含量分别为 (54.7± 13 .4) %和 7.64± 3 .15,与癌周正常粘膜组织比较具有显著差异 (P<0 .0 1) ;随着 Dukes分期的进展 ,PCNA标记指数和 DNA相对含量逐渐增加 ,C、D期较 A、B期有显著差异 (P<0 .0 1)。结论 细胞增殖核抗原及 DNA倍体水平不同的表达与结肠癌的发生发展有明显的关系 ,两者可以作为结肠癌临床分期和预后的监测指标之一。
ObjectiveTo investigate the relationship between the e xpression of proliferating cell nuclear antigen(PCNA) and DNA ploidy and the cli nical stages in colon carcinoma.MethodsImmunohistochemical sta ining and computer-assisted image analysis were used to detect index of PCNA a nd nuclear DNA content in 65 cases of colon carcinoma and normal colon mucosa.ResultsIndex of PCNA and nuclear DNA content in colon carcinoma we re (54.7±13.4)% and 7.64±3.15 respectively, which were significantly differe nt as compared with those of normal colon mucosa(P<0.01). With the advanc ement of Dukes stages, the detect index of PCNA and nuclear DNA content increase d gradually, whose differences were all significant(P<0.01)in comparing C 、D stages with A、B stages.ConclusionThe different expression of PCNA and DNA ploidy are obviously related to the tumorigenesis and progres sion of colon carcinoma. Both would become one of the prognostic indicators for colon carcinoma.
出处
《新乡医学院学报》
CAS
2004年第1期17-18,22,共3页
Journal of Xinxiang Medical University
