摘要
以甜菜渣、果胶、海藻酸钠为原料 ,与表氯醇在碱性条件下交联 ,制备了果胶酶亲和吸附剂并应用这几种亲和吸附剂对草酸青霉果胶酶和黑曲霉果胶酶进行亲和层析实验。结果表明 :用小于 60目的甜菜渣粉末和海藻酸钠制备的果胶酶亲和吸附剂 (分别简写为SBP吸附剂和SA吸附剂 )具有较好的纯化果胶酶性能 ,其中SBP吸附剂性能稳定 ,而SA吸附剂膨胀系数较大 。
Suger beet pulp (SBP)powder, pectin and sodium alginate (SA) were cross\|linked with epichlorohydrin respectively in an alkaline condition.Such preparations were used as the affinity chromatographic adsorbents for the separation and purification of pectinase from Penicillium oxalicum and Aspergillus niger. The results showed that the affinity chromatographic adsorbent prepared by SBP powder with less than 60 sieve mesh has better character than others for pectinase purification. SBP adsorbent was stable, while SA adsorbent resulted in unstable flow of mobile phase because its larger dilatability.;
出处
《中国生物工程杂志》
CAS
CSCD
2004年第1期70-73,共4页
China Biotechnology
基金
国家"8 63"计划资助项目 ( 2 0 0 1AA2 460 14 )
科学院知识创新工程项目 (KZCX1 SW 19)
关键词
甜菜渣
海藻酸钠
亲和吸附剂
果胶酶
发酵法
Suger beet pulp Sodium alginate Affinity chromatographic adsorbent Pectinase
