摘要
用Alcalase水解酪蛋白,用TNBS法监测酪蛋白的水解度(DH),运用纳滤技术改善产品质量;同时还研究了不同rN/rP(摩尔比)的CPP(酪蛋白磷酸肽)结合钙离子和亚铁离子的能力.结果表明,用Alcalase酶可以使酪蛋白的水解度(DH)达到16%～20%,TNBS法可以明显减少碱的引入,从而降低CPP产品的灰分含量.截留相对分子质量为150的纳滤膜可以适当的降低CPP产品的rN/rP,改善产品质量,而且能达到80%的总氮回收率;在DH为20%左右,rP基本上没有损失.该工艺适合于工业化生产较高rN/rP的CPP产品.rN/rP较低的CPP结合钙的能力好于rN/rP较高的CPP产品;CPP也可以抑制亚铁离子的沉淀.
Alcalase was employed in hydrolyzing the casein and TNBS method was used to detect the degree of hydrolysis (DH)during the hydrolysis of the casein. Nanofiltration was also used in the procedure to produce CPP with better quality by removing short peptides and free amino acids in the casein hydrolysates. Calcium and iron(Ⅱ)binding ability of CPP was also studied in this paper. The results showed that the DH of casein reached 16%～20% by using the Alcalase compared with 12% DH by trypsin. The method of TNBS decreased the addition of base to the solution thus the content of ash could be reduced. The nanofiltration membrane with 150 Dalton of molecular weight interception reduced the molar ratio of N to P (r_N/r_P), improved the quality of the product ,and the nitrogen recovery rate of 80% could be reached. P could be completely recovered at DH 20%. Hence, the technology is suitable to produce CPP products with high r_N/r_P(molar ratio) in a large scale. CPP with lower r_N/r_P(molar ratio)was found to be better than CPP with higher r_N/r_P(molar ratio) in binding calcium and inhibiting precipitation of calcium phosphate. CPP was also found to be able to inhibit the precipitation of Iron(Ⅱ).
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2004年第1期89-93,共5页
Journal of Wuxi University of Light Industry
