摘要
对红细胞进行蛋白酶处理、戊二醛固定,采用pH7.2等渗磷酸盐作为缓冲液,用光径0.5cm比色池测定凝集活性,读取测定终点的时间以2h为宜.建立的检测方法简单易行,得出的结果具有良好的灵敏度及重现性.
The procedure for determination of hemagglutination activity(HA) of wheat germ agglutinin(WGA) was improved. Major improvements include: 1). the self sedimentation of erythrocytes was deducted in the definition and calculation of HA; 2). erythrocytes were sensitized with trypsin and fixed with glutaraldehyde prior to HA tests; 3). pH 7.2 isotonic phosphate buffered saline was used as the working liquid; 4). UV absorption of erythrocyte suspensions was taken using 0.5 cm path-length cells; and 5).appropriate end-point was determined as 2 hours after adding WGA into the suspension. The protocol thus derived was proved to be convenient, capable of having good sensitivity and reproducibility.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2004年第1期49-53,共5页
Journal of Wuxi University of Light Industry
