摘要
目的:观察实施加速度作用后造成大鼠脑缺血,在急性期血浆中的一氧化氮、一氧化氮合酶(NOS)和白细胞介素-6(IL-6)的含量变化,探讨加速度对机体引起的继发性脑损伤程度及机制。方法:30只雄性健康wistar大鼠,随机分为3组,分别施加+1Gz(正加速度)、高+Gz和正、负加速度(±Gz)的交替作用,并把被施加+1Gz加速度的大鼠作为对照组。于加速度作用后即刻麻醉,腹主动脉采血。分别测定各组大鼠血浆中一氧化氮、NOS和IL-6的含量。结果:一氧化氮含量:±Gz交替组犤(60.4±6.6)μmol/L犦与高+Gz组犤(50.3±2.2)μmol/L犦比较,t=16.5250;高+Gz组与对照组犤(35.9±3.3)μmol/L〗比较,t=27.9397;±Gz交替组与对照组比较,t=50.0324,P均<0.01。NOS活性:±Gz交替组犤(890±73)μmol/(s·L)犦与高+Gz组犤(791±60)μmol/(s·L)犦比较,t=3.3147;高+Gz组与对照组犤(332±27)μmol/(s·L)犦比较,t=23.4389;±Gz组与对照组比较,t=25.9615,P均<0.01。IL-6含量:±Gz交替组犤(132±18)ng/L犦与高+Gz组犤(106±15)ng/L犦比较,t=19.6748;高+Gz组与对照组犤(71±10)ng/L比较,t=24.9296;±Gz组与对照组比较,t=48.1645,P均<0.01。结论:正、负加速度交替和单纯高正加速度作用均可使血浆中的一氧化氮、NOS和IL-6的含量增加明显,且正。
AIM:To observe the changes of nitric oxide(NO),nitric oxide synthase(NOS) and interlukin(IL) 6 contents in rats with acute cerebral ischemia induced by acceleration so as to investigate the effect and mechanism of acceleration on secondary brain injury. METHODS:Thirty male healthy Wistar rats were equally divided into three groups at random:control group,high+Gz group and±Gz group.Rats were anaesthesised to collect blood in order to detect the contents of NO,NOS and IL 6 just after acceleration. RESULTS:The contents of NO were(60.4±6.6)μmol/L for the ±Gz group and(50.3±2.2)μmol/L for the high+Gz group,with a significant difference(t=16.525 0, P< 0.01).As compared with the control group[(35.9±3.3)μmol/L],the contents of NO in the high+Gz group and±Gz group were significantly higher(t=27.939 7,P< 0.01;t=50.032 4,P< 0.01).The activities of NOS were(890±73)μmol/(s·L)for the±Gz group and(791±60) μmol/(s·L)for the high+Gz group and there was a significant difference between the two group(t=3.314 7,P< 0.01),while both of which were significantly higher than that of the control group[(332±27)μmol/(s·L)](t=25.961 5,P< 0.01; t=23.438 9, P< 0.01).The contents of IL 6 were(132±18) ng/L for the ±Gz group,(106±15) ng/L for the high+Gz group and(71±10) ng/L for the control group,and a difference was significantly showed among the three groups(t=19.674 8,48.164 5,24.929 6,P< 0.01). CONCLUSION:±Gz and+Gz alone lead to the contents of NO, NOS and IL 6 on the increase and the±Gz has a better impact on the severity of brain injury.
出处
《中国临床康复》
CSCD
2003年第31期4182-4183,共2页
Chinese Journal of Clinical Rehabilitation
基金
2001全军医药卫生科研基金课题"十五"面上项目(01MA202)~~
