摘要
为了观察急性重度低压缺氧对心肌脂质过氧化及心肌酶学的影响，对３９只成年健康Ｗｉｓｔａｒ大鼠急性低压缺氧实验。结果发现，急性缺氧后心肌组织中硒谷胱甘肽过氧化物酶（ＳｅＧＳＨｐｘ）活性明显降低，丙二醛（ＭＤＡ）含量减少，ＳｅＧＳＨｐｘ／ＭＤＡ比值下降。结果提示，急性低压缺氧后，心肌组织中的脂质过氧化反应并无明显增强，仅有心肌的潜在抗氧化能力降低。另外，急性低压缺氧后，心肌酶活力显著增高，说明急性低压缺氧对心肌有一定损伤作用。鉴于心肌脂质过氧化反应无明显增强，提示心肌损伤与脂质过氧化无明显相关。心肌酶活力增高的原因，可能是急性缺氧对心肌细胞直接作用的结果。
The damage caused by acute hypobaric hypoxia
upon myocardium is apotential danger for pilots flying in high altitude. With the advance of the
investigation ofmyocardial ischemia-reperfusion injury , it was found recently that the increase
of oxygenfree radical production and the strengthening of lipid peroxidation reaction were
importantcauses for the development of ischemic myocardial damage.The development and
the progress of atherosclerosis were also closely related to the damage from lipid
peroxidation. In this article , SeGSHpx , MDA content , SeGSHpx/MDA ratio and several kinds of
my-ocardial enzymes such as CPK,CK-MB,LDH,α-HBD and AST were measured in 39 healthy
adult Wistar rats staying at 7500m for 30min in a hypobaric chamber in order toobserve the
effect of acute hypoxia upon the myocardium and the relationship between lipid peroxidation
and myocardial dalnage. The results showed that activity of myocardialenzymes was raised
significantly after acute hypoxia,the drference was remarkable ascompared with the control
values(P<0.01 or 0.00 1). Acute hypobaric hypoxia had al-so some infiuences on the
myocardial SeGSHpx activity , MDA content , and SeGSHpx/MDA ratio. Normal organism has
the ability to combat peroxidation with sufficient oxy-gen supply , the human body can
generally tolerate the influence of oxygen free radicalsupon myocardiai tissue by the dynamic
balance of the production of intracellular peroxi-dates and the protective mechanism of the
antioxidant system. The change of the activity of SeGSHpx in rats in the hypoxic group were
much lower than that in the control group,the difference was very significant(P<0.001) which
indicates the inefficiency of my-ocardium against peroxidation. MDA is the end product of lipid
peroxidation , and the ele-vation of plasma MDA content reflects that the lipid peroxidation of
the body is exacer-bated. In this article , the myocardial content of MDA of rats in the hypoxic
group waseven lower than that in the control group , the difference between the two groups was
sig-nificant(P<0.05). The results suggested that there was no exacerbation of lipid peroxi-dation
after severe hypoxia.SeGSHpx / MDA ratio reflects the combined change of the above two
factors. Myocardial SeGSHpx / MDA ratio of rats in the hypoxic group was lower than that in the
control group , and the difference was very significant(P<0.01), suggesting the lowering of
potential ability of myocardium to combat peroxidation.To sum up , the authors considered that
there was no intimate relationship between myocar-dial damage and lipid peroxidation. The
cause for the elevation of the activity of myocar-dial enzymes might be the direct result of
acute hypoxia upon myocardial cells。
出处
《航天医学与医学工程》
CAS
CSCD
1994年第3期181-185,共5页
Space Medicine & Medical Engineering
关键词
急性高空缺氧
心肌
过氧化脂质类
acute altitude hypoxia , myocardium ,
glutathione peroxidase , lipid per-oxides
