摘要
以伯氏疟原虫K_(173)株,小鼠体内试验系统,采用剂量递增连续3天给药法,历时420天,连续血传60代,培育出高度抗氯喹的RC/k173系,其抗性指数大于100.以维持剂量继续血传60代或经冷冻保存360天,其抗性强度仍保持在原来水平.但停药血传到第10代,抗性基本消失.抗药系原虫的毒力较亲代系低.对7种常用抗疟药敏感性测试的结果表明,该系除对乙胺嘧啶、周效磺胺和伯喹敏感外,对另外4种药物均显示出程度不等的交叉抗药性.推荐该品系可作为新抗疟化合物与氯喹交叉抗性研究的模型,并以给药血传或冷冻保存作为保种方法.
Under gradually increasing drug pressure Plasmodium berghei K_(173) strain developed into a highly chloroquine-resistant line.After 60 passages over a period of 420 days,its resistance became stable and no change occurred even though maintained with small dose of chloroquine or under cryopreservation for 360 days.But in drug-free condition,it regained sensitivity to chloroquine after 10 passages in mice.Its resistant index is higher than 100,yet its virulence is lower than the normal line.The susceptibility of this chloroquine-resistant line to 7 antimalarials revealed more sensitive to pyrimethamine,sulfadoxine and primaquine,but exhibited some degree of cross resistance to the other 4 drugs,i.e. mefloquine,quinine,qinghaosu and pyronaridine.Therefore,we consider that this line (RC/k173) may be used as a valuable model for evaluating new antimalarials in the study of the possible cross-resistance to chloroquine.
出处
《军事医学科学院院刊》
CSCD
北大核心
1989年第2期118-121,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
疟原虫
抗疟药
抗性培育
Plasmodium berghei
antimalarials
development of resistance
resistant index
