摘要
目的 :研究卵巢上皮癌中CD44基因mRNA内含子 9的滞留情况及其临床意义。方法 :采用半定量RT PCR方法检测不同卵巢组织中CD44基因mRNA内含子 9的滞留率及相对含量。结果 :内含子 9在正常卵巢组织中无滞留 ;在卵巢良性、交界性、恶性上皮性瘤中滞留率及相对含量分别为 3 6.67%和 8.3 7% ,87.50 %和86.87% ,93 .3 3 %和 91.17% ;恶性和交界性上皮性卵巢瘤中内含子 9的滞留率及相对含量明显高于良性上皮性卵巢肿瘤 (P <0 .0 1) ;不同组织学类型和临床分期内含子 9的滞留率和相对含量无显著性差异 (P >0 .0 5)。结论 :检测内含子
Objective: To investigate unusualretenti on ofintron 9 in CD44 gene transcripts in ovarian epithelial cancer and its cli nical significance. Methods: Semi-quantitatively reverse tran scription-polymerase chain reaction (RT-PCR) was used to analyzethe retentio n rate and relativecontent of intron 9 in CD44 gene transcripts in 30 cases ofmalignant ovarian epithelialcancer , 8 of ovarian borderline tumors, 30 of be nign ovariantumorsand 10 ofnormal ovary.Results: The r etention of intron 9 in CD44 gene transcripts was not foundin normal ovarian t issue.The retentionrates and relative contents of intron 9 in ovarian benign , borderline, and malignant tumor were 36.7%and 8.37%, 87.50% and 86.87%, 93 .33% and91.17%,respectively. The retention of intron 9 in ovarian borderlineand malignant tumors were significantly higher than that in benign tumors (P <0.01).There was similar retentionof intron 9 in different histological type s and clinical stages ofovariantumors. No correlation was found between theclinical stages or histological types of ovarian tumors and the retention of int ron 9 (P>0.05).Conclusion: Detection of retention ofin tron 9 may be a molecular diagnostic tool for ovarian carcinoma.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2004年第1期86-88,共3页
Journal of China Medical University
