摘要
目的 :探讨核因子κB(NF κB)在大鼠双侧后肢缺血再灌注 (I/R )致肺损伤中的表达及意义。方法 :建立大鼠双侧后肢I/R模型 :48只大鼠随机分为假手术组 (Ⅰ组 ,n =8)、缺血组 (Ⅱ组 ,n =8)、缺血再灌注组 (Ⅲ组 ,n =3 2 )。Ⅱ组在缺血 4h末 ,Ⅲ组分别于再灌注 4,12 ,2 4,48h切取双肺 ,应用半定量RT PCR和Westernblot方法检测肺组织NF κBp65/IκBβ的mRNA和蛋白产物表达。结果 :Ⅲ组各时点NF κBp65mRNA表达较Ⅰ、Ⅱ组增加 ,再灌注后 12h达高峰。p65蛋白产物表达亦逐渐增加 ,12h达高峰 ,持续到术后 48h ;IκBβ的mRNA和蛋白表达在I/R后同样开始增加 ,2 4h的表达量最多。结论 :NF
Objective: To investigate the effect of sk el etal muscle ischemia/reperfusion on the expression of nuclear factor kappa B(NF -κB) in lung injury of rats. Methods: Rat models of hind lim bs ischemia/reperfusion were established. Forty-eight rats were divided into 3groups: sham operation group(groupⅠ, n=8), ischemia group(groupⅡ, n=8),ischemia/reperfusion injury group(groupⅢ, n=32), which included subgroup s of 4, 12, 24, 48 hours after reperfusion. The changes of mRNA and protein expr ession of NF-κB p65/ IκBβ in lung were determined by using RT-PCR or Wester n blot. Results: In group Ⅲ, NF-κB p65 mRNA increased quick ly after reperfusion and reached peak 12 hours after reperfusion. The p65 detect ed by using Western blot reached peak 12 hours after reperfusion and then decrea sed slowly, but still remained at high level 48 hours after reperfusion. Express ion of IκBβ mRNA and protein in lung also increased after reperfusion and reac hed peak 24 hours after reperfusion. Conclusion: The NF-κBp6 5/IκBβ may play an important role in the damage of lung function after skeleta l muscle ischemia/reperfusion injury in rats.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2004年第1期9-11,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目 ( 30 371 40 1 )
关键词
核因子ΚB
缺血再灌注
肺损伤
nuclear factor kappa B
ischemia/reperfusion in jury
lung injury
