摘要
利用水平切片淀粉凝胶电泳技术 ,分析了不同蝗区东亚飞蝗四个地理种群的遗传结构。在检测的 2 0个酶基因座位中 ,四个种群均表现出一定的遗传多态性 ,多态位点的百分率普遍偏高 (P =70 %～ 80 % ) ,但由于杂合子数目较少而使每个位点的平均杂合度观察值偏低 (Ho=0 0 2 3～ 0 0 3 2 )。对每个基因座位的各基因型进行 χ2 检验 ,除Adk_1、Gdh_1、G3pd_1和Pgm_1在部分种群符合Hardy_Weinberg平衡外 ,其余绝大多数基因座位的基因型频率显著偏离Hardy_Weinberg平衡。从F统计量看 ,四个种群之间的遗传分化较低 (Fst=0 0 60 6)。它表明 :东亚飞蝗较强的长距离迁飞行为增加了种群之间的基因交流 ,降低了种群之间的遗传分化。根据Nei的遗传一致度 (I)和Roger的遗传距离 (D)进行分析 ,在山西临猗与山西永济 (I=0 964,D= 0 175)、河南中牟与江苏沛县种群 (I =0 957,D =0 160 )之间 ,呈现出较高的遗传一致度和较小的遗传距离。结果表明
The genetic structure of the four geographic populations of the Orient al migratory locust Locusta migratoria manilensis was analyzed using horizon tal starch gel electrophoresis. Among 20 loci of 13 isozymes identified, the higher the percentage of polymorphic loci (P=70%-80%), the lower the observed over all heterozygosity (H_o=0.023-0.032). The result from Chi_square test fo r the genoty pe frequencies showed the genotype frequency at most loci deviated significantly from Hardy_Weinberg equilibrium except Adk_1, Gdh_1, G3pd_1 and Pgm_1. Based on F_statistics (average F_(st)= 0.0606), an extremely small g enetic differentiation among the four populations was observed. It appeared that the long_distance mig r ation of the locust enhanced gene flow and decreased genetic differentiation. Th e divergence among four populations was revealed using Nei’s genetic identity ( I) and Roger’s genetic distance (D). The higher the genetic identity, t he small er the genetic distance observed between Shanxi_Linyi and Shanxi_Yongji populati ons (I=0.964, D=0.175), and between Jiangsu_Peixian and Henan_Zhongmou p opulations (I=0.957, D=0.160). The results indicated that there was a positive relationship between genetic differentiation and geographic distance.
出处
《昆虫学报》
CAS
CSCD
北大核心
2004年第1期73-79,共7页
Acta Entomologica Sinica
基金
国家自然科学基金项目 ( 30 1 70 6 1 2 )
陕西省科学技术委员会资助项目 ( 0 1 2 0 1 5)
关键词
东亚飞蝗
种群
遗传分化
等位酶
中国
Locusta migratoria manilensis
population
genetic differentiation
allozyme
China
