摘要
背景与目的:透明质酸(hyaluronan,HA)是由多种细胞(包括肿瘤细胞)分泌的细胞外基质(extra-cellularmesenchyma,ECM)的主要成分之一,它参与细胞的定位、迁移,并涉及肿瘤细胞的运动与增殖,已有研究发现实体肿瘤的进展过程中存在HA代谢失衡,并认为HA及其配体的作用与肿瘤的侵袭转移和预后有关。本研究旨在探讨细胞粘附分子CD44及其主要配体HA与卵巢腺癌病理类型的关系。方法:对78例卵巢腺癌、22例卵巢腺瘤和2例交界性粘液性腺瘤的组织病理标本进行HA和CD44免疫组化检测,测定在良、恶性及不同组织分级肿瘤中的表达,其结果分别按阴性、弱阳性及强阳性记录。结果:HA主要表达于肿瘤间质和细胞外基质,细胞膜和胞浆偶有染色。在组织切片上癌巢周边的间质表达更强。在102份标本中,肿瘤间质HA阳性占71.57%,其中在恶性肿瘤的阳性率是83.33%,强阳性占41.03%;而在良性肿瘤中的阳性率为27.27%。肿瘤间质HA的表达随肿瘤组织分级升高而增强,在浆液性癌的强阳性率高于其他病理类型,肿瘤间质HA表达与肿瘤细胞CD44表达有显著相关性,与组织分级的相关性优于CD44;但HA在肿瘤细胞内的表达率低,且与病理类型、组织分级及CD44表达无关。结论:恶性肿瘤组织间质高表达HA,与肿瘤组织分级及病理类型有关。
BACKGROUND &OBJECTIVE: Hyaluronan (HA) is one of the major components of extra cellular mesenchyma (ECM), which is secreted by several kinds of cells, including tumor cells. HA plays a role in cellular location and proliferation. It also takes part in the tumor cell motion and proliferation. It has been found that there was HA metabolism imbalance during the development of the solid tumors, and that, HA and its ligands might be related to tumor invasion, metastasis, and prognosis. The purpose of our research was to investigate the relationship among HA, the major ligand of cell adhesive molecule CD44, and pathologic types of ovarian adenocarcinoma. METHODS: HA and CD44 immunohistochemical assays were performed in the histopathologic specimens of 78 cases with ovarian adenocarcinoma and 24 cases with ovarian adenoma. Their expression in benign and malignant tumors with various histopathologic gradations were determined. Results were recorded as negative, weak positive, and strong positive. RESULTS: HA is mainly expressed in tumor stroma and extra cellular mesenchyma, while cell membrane and cytoplasm were stained occasionally. In histologic sections, stronger expression was found in tumor stroma peripheral to carcinomatous nests. In total 102 specimens, positive HA expression in tumor stroma accounted for 71.27%. The positive rate in malignant tumor was 83.33%; the strong positive rate was 41.03%. However, the positive expression rate in benign tumor was 27.27%. The level of stromal HA expression increased along with histologic gradation. The expression level in serous adenocarcinoma was higher than that in other pathologic types. Expression of HA in stroma and expression of CD44 in the tumor cells were significantly correlated; and the correlation of HA expression with histologic gradations was better than that of CD44. However, the expression of HA in tumor cells was low and unrelated to pathologic types, histopathologic gradations, and CD44 expression. CONCLUSION: High level of HA in the stroma of ovarian adenocarcinoma is related to tumor histological gradations and pathologic types.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2004年第2期177-180,共4页
Chinese Journal of Cancer
基金
福建省科技厅科技项目(No.99-Z-160)~~
