摘要
目的 探讨麻风病患者外周血相关免疫分子的变化和临床意义。方法 应用流式细胞分析仪 ,以荧光标记的鼠抗人单克隆抗体对 16例麻风病患者外周血淋巴细胞CD3 /CD4/CD8、CD3 /CD(16+ 56) (NK)和CD3 /HLA -DR进行测定。结果 16例患者发病初期外周血淋巴细胞亚群中CD3 + /CD4+ 、CD3 + /HLA -DR-检测值和CD4/CD8比值均分别低于健康组相应参数的下限 ,CD3 + /CD(16+ 56) + 和CD3 + /HLA -DR+ 检测值分别高于健康组相应参数的上限、NK的检测值在正常范围内。 13例患者治疗病情稳定后 2-4周 ,CD3 + /CD4+ 、CD3 + /HLA -DR-的检测值和CD4/CD8比值均逐步增高 ,CD3 + /CD(16+ 56) + 、CD3 + /CD8+ 和CD3 + /HLA -DR+ 稍有增高或变化不明显 ,NK的检测值稍有增高 ;3例患者病情波动时CD3 + /CD4+ 、CD3 + /HLA -DR-检测值和CD4/CD8比值较稳定期明显下降 ,而活化T淋巴细胞CD3 + /CD(16+ 56) + 、CD3 + /HLA -DR+ 和NK细胞的检测值较稳定期明显增高 2倍以上。结论 通过外周血相关免疫分子的动态观察 ,有助于检测病情变化 ,对及时治疗和评价疗效有重要意义。
Objective To investigate the relationship between leprosy and some correlative immunomolecules in peripheral blood.Methods lymphocytes from peripheral blood in 16 leprosy patients in different periods were immunologically labeled by monoclonal antibodies from mouse CD 3/CD 4/CD 8,CD 3/CD (16+56) (NK) and CD 3/HLA-DR were determined by using flow cytometry(FCM).Results The levels of CD 3 +/CD 4 +,CD 3 +/HLA-DR and CD 4/CD 8 ratio in active period were lower in 16 patients than those of normal group,while CD 3 +/CD (16+56) +,CD 3 +/HLA-DR + were higher,NK was in the normal range.In stable period 2～4 week after treatment CD 3 +/CD 4 +,CD 3/HLA-DR - and CD 4/CD 8 were gradually increased,CD 3 +/CD (16+56) +,CD 3 +/CD 8 + and CD 3 +/HLA-DR + were increased slightly or no change ,and NK was increased slightly in 13 cases in unstable period.In other 3 case,CD 3 +/CD 4 +,CD 3 +/HLA-DR and CD 4/CD 8 were decreased than that in stable period,while the CD 3 +/CD (16+56) +,CD 3 +/HLA-DR + and NK were double increased than that in stable period.Conclusions Dynamic determination of associated immunlogical molecules in peripheral blood of leprosy patients is helpful to evaluate the change of illness state,it also play an important role in the treatmant of the disease.
出处
《中国医师杂志》
CAS
2003年第7期909-910,914,共3页
Journal of Chinese Physician
