摘要
目的:动态观察慢性乙型肝炎患者细胞因子诱导的杀伤(CIK)细胞的增殖及杀伤活性。方法:抽取10例健康人及20例慢性乙型肝炎患者的外周血,常规分离单个核细胞(PB-MC),加IFN-γ、IL-2及抗人CD3单克隆抗体(mAb)后培养,诱导CIK细胞形成。于培养后3、6、12、24及30 d,取培养的 细胞,用流式细胞仪检测CIK细胞表面CD3、CD4和CD8以及CD4、CD25、CD3、CD56和CD95、CD28分子的表达水平、增殖及杀伤活性。结果:慢性乙型肝炎患者CIK细胞的增殖及杀伤活性均低于正常对照组。培养不同时间乙肝患者CIK细胞的增殖倍数、杀伤活性和上述表面标志的表达水平,均较培养前明显增高,于培养后12 d达高峰。结论:慢性乙型肝炎患者CIK细胞的增殖倍数、杀伤活性均低于正常人,但明显高于培养前;这可能是导致HBV感染持续发展的原因之一。
AIM: To oberve dynamically proliferation and cytotoxicity of cy-tokine-induced killer cells (CIKs) in peripheral blood from patients with persistent infection of hepatitis B virus. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated routinely from 10 normal volunteers (control group) and 20 patients with chronic hepatitis B. PBMCs were co-cultured with IFN-γ, rhlL-2 and anti-CD3 mAb to induce CIK cell generation. The expressions of single staining CD3, CD4, and CD8, as well as double staining CD4 CD25, CD3 CD56 and CD28 CD95 on CIK cells were analyzed by flow cytomctry on 3,6,12,24 and 30 days of co-culture. RESULTS: The prolif-erative level and killer activity of CIK cells from the patients were lower than those from control group. The proliferative multiple, killer activity and expressions of above surface markers on CIK cells from the patients at various times after co-culture were higher than those before co-culture, and reached peak values on 12 days after co-culture. CONCLUSION: The proliferative multiple and killer activity of CIK cells from the patients are lower than that of normal people, which may be the reason of persistent development of HBV infection.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第6期554-556,共3页
Chinese Journal of Cellular and Molecular Immunology
