摘要
纸片荧光法是利用细菌产生某些代谢酶或化谢产物的特点,而建立的一种酶-底物反应法。该方法比AOAC规定的方法节省时间,而且将待测细菌所需的营养成分、酶促底物以及抑制杂菌的成分均固相化在纸片上,进一步简化了实验准备、便于携带,更适用于HACCP的现场监管。本实验针对纸片荧光法的灵敏度、特异性、准确性、定量反应、对受伤菌种恢复作用及对大肠杆菌的准确性进行了研究。该方法的定性、定量结果与AOAC方法无差异,检验受伤菌及大肠杆菌的结果也与AOAC法相一致,未见非特异性干扰。纸片法作为一种独特、方便、准确的快速检测法值得推广。
A rapid and specific detection method for total coliforms and E.coli in frozen foods was developed based on B-galactosidase and B-glucuronidase activity of the bacterium. The materials about specific indication-nutrirent、enzymaticsubstrate、antibacterical component were fixed on the designed paper slip. If Coliform or E. coli were present in the foodsample, the solution would cause fluorescence under UV lamp, without the need for comfirmatory or complete tests. Thedetecting results were very to interpret. The mehtod which was less costly、less labor needed than the standard methods(AOACmehtods; ISO mehtods) and easy to carry out should be better used for HACCP in situ.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第1期135-139,共5页
Food Science
