摘要
应用单克隆抗体一胶体金标记技术,采用徒手切片法和斑点试验法检测柑桔黄龙病获得成 功。该方法检测速度快,操作简单,成本低,适合柑桔种苗大批量检测和田间普查。采用单抗金标技术 对田间栽培的柑桔品种、柑桔实生苗、试管微芽嫁接苗、热处理脱毒后的嫁接苗进行检测,结果表明 田间栽培的6个柑桔品种不论是否显示黄龙病症状均检测到柑桔黄龙病病菌,而消毒种子播种所得 实生苗及试管微芽嫁接苗为无病株,带病株经过30d 40℃恒温热处理后产生的新梢及其嫁接苗绝大 部分也是无病株。
The research had succeeded in detecting the Citrus Huanglongbing through the hand-sliced method or dot-marked observation by the monoclonal antibody with colloidal gold-labeled （MACG） technique. The methods are suitable for Citrus seedling detection of Huanglongbing pathogen in batch and general investigation in field, for they are rapidly and easily manipulated and low in price. By the MACG technique, we detected the Citrus plants in field, Citrus seedlings, their microbud-grafting plantlets and the plants after heat-therapy processed, the result showed that 6 kinds of Citrus plant in field, no matter the symptom of Huanglongbing pathogen was appeared or not, were detected to be infected by Huanglongbing pathogen. However microbud- g plantlets in tube and the seedlings, which grew from sterilized Citrus seeds, were Huanglongbing pathogen-free. Even most of the new branches and theirsgrafted plants which came from plants-infected after stable temperature processed for 30 days in 40℃ had no Huanglongbing pathogen.
出处
《中国农学通报》
CSCD
2005年第12期336-338,共3页
Chinese Agricultural Science Bulletin
基金
汕头市重点科技项目资助
关键词
柑桔黄龙病
单克隆抗体
胶体金
快速检测
Citrus Honglongbing, Monocolony antibody, Colliodial gold labeled, Rapid detection
